Ning Wan

Dr. Wan earned her Ph.D. in Pharmacokinetics from China Pharmaceutical University from September 2017 to June 2022. She conducted postdoctoral research at China Pharmaceutical University from June 2022 to November 2025, and has been an Associate Research Fellow at the same institution since November 2025. Dr. Wan has long been engaged in chemical proteomics research in the field of “small molecule–protein interactions”, focusing on the complex binding modes between active molecules and target proteins. She has achieved the following innovative results in the development of proteomics-based target discovery technologies: (1) Developed a characteristic ion diagnostic technology that bypasses antibody dependence, enabling highly sensitive mining of low-abundance covalent modification targets in public databases (Nat Methods, 2022), which received a simultaneous commentary in Nat Methods and a recommendation from Faculty Opinions; (2) Pioneered TRAP (target-responsive accessibility profiling), a chemical modification-free technology that utilizes protein accessibility changes to characterize conformational dynamics, enabling precise identification of weak/transient non-covalent interactions in complex systems (Nat Chem Biol, 2023), overcoming the limitations of traditional probe-dependent approaches; (3) Proposed a strategy integrating subcellular fractionation with modificomics, revealing the spatial heterogeneity of lysine lactylation (J Am Soc Mass Spectrom, 2024). These studies provide an original technology platform for active molecule target discovery and functional elucidation, combining methodological innovation with significant biological application value.

Selected publications:
1. Wan N#, Wang N#, Yu S, Zhang H, Tang S, Wang D, Lu W, Li H, Delafield DG, Kong Y, Wang X, Shao C, Lv L, Wang G, Tan R, Wang N, Hao H, Ye H*. Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome. Nature Methods, 2022, 19(7): 854-864.
2. Wan N, Liu C, Hao H, Ye H. Emerging trends in chemoproteomics: Mapping the landscape of protein-metabolite interactions. Current Opinion in Chemical Biology, 2025, 89: 102631.
3. Tian Y#, Wan N#, Zhang H#, Shao C#, Ding M, Bao Q, Hu H, Sun H, Liu C, Zhou K, Chen S, Wang G, Ye H, Hao H. Chemoproteomic mapping of the glycolytic targetome in cancer cells. Nature Chemical Biology, 2023, 19(12): 1480-1491.
4. Bao Q#, Wan N#, He Z, Cao J, Yuan W, Hao H, Ye H. Subcellular Proteomic Mapping of Lysine Lactylation. Journal of the American Society for Mass Spectrometry, 2024, 35(12): 3221-3232.