张丽华 Lihua Zhang

研究领域：
1. 研制高新型样品预处理和分离材料
针对蛋白质组等复杂样品的分析需求，着重研制新型整体材料、聚合物颗粒、硅胶颗粒等，并对其表面进行功能修饰，以实现样品的高效低残留的预处理和分离。
2. 发展蛋白质组定量新方法新技术
发展基于色谱，以及一级/二级质谱的标记和非标记的相对和绝对定量新方法，提高规模化蛋白质定量的准确度和精密度。
3. 构建集成化分析平台
发展蛋白质变性、还原、酶解、标记等技术，并与微纳升级液相色谱-质谱联用，构建集成化蛋白质定性和定量分析平台，为实现高通量、高精密度和高准确性的规模化蛋白质分析提供关键技术支撑。

Research Areas:
1. Development of advanced sample pretreatment and separation materials
Addressing the analytical needs of complex samples such as proteomes, focus on developing novel monolithic materials, polymer particles, silica gel particles, etc., and functionally modifying their surfaces to achieve efficient and low-residue sample pretreatment and separation.
2. Development of new methods and technologies for proteome quantification
Develop new labeled and label-free relative and absolute quantification methods based on chromatography and primary/secondary mass spectrometry to improve the accuracy and precision of large-scale protein quantification.
3. Construction of integrated analysis platforms
Develop protein denaturation, reduction, enzymatic digestion, labeling and other technologies, and integrate them with micro-nano-liter liquid chromatography-mass spectrometry to construct integrated protein qualitative and quantitative analysis platforms, providing key technical support for achieving high-throughput, high-precision and high-accuracy large-scale protein analysis.

Wang, S., Zhang, Z., Yang, K., Zhao, J., Zhang, W., Wang, Z., Liang, Z., Zhang, Y., Zhang, Y., Liu, J., & Zhang, L. (2024). SMMP: A Deep-Coverage Marine Metaproteome Method for Microbial Community Analysis throughout the Water Column Using 1 L of Seawater. Analytical chemistry, 96(29), 12030–12039. https://doi.org/10.1021/acs.analchem.4c02079